Perfusion-Based Recellularization of Rat Livers with Islets of Langerhans

Abstract Purpose Artificial organs might serve as alternative solutions for whole organ transplantation. Decellularization of a liver provides non-immunogenic matrix with the advantage three afferent systems, portal vein, hepatic artery and bile duct. This study aims to evaluate recellularization rat livers islets Langerhans via duct vein comparison different perfusion routes. Methods Rat were decellularized in pressure-controlled manner repopulated intact isolated either or Results Repopulation showed islet clusters stuck within vascular system demonstrated by ellipsoid borders thick reticular tissue around cluster Azan staining. After duct, distributed close vessels parenchymal space without surrounding layer. Large had diameter up 1000 µm clear shapes. Conclusion We be superior repopulation Langerhans. technique may bioengineering platform generate an implantable functional endocrine neo-pancreas provide scaffolds anatomic benefit systems facilitate co-population cells.